Yukiko Tsukuba (June 10, 1906 – June 8, 1977) was a Japanese actress on stage, in silent films, and in early sound films. She was also the All-Japan women's billiards champion in 1929.

Tsukuba was born in Tokyo. She trained as a geisha, and became an internationally publicized beauty and film star while she was still in her teens. Tsukuba began her screen career at the Shochiku studio, working with directors including Yasujiro Shimazu, Hiroshi Shimizu, Yoshinobu Ikeda, Heinosuke Gosho, Kiyohiko Ushihara, Buntaro Futagawa, Torajiro Saito, and Mikio Naruse.